Gallery

Antirrhinum majus - leaf vasculature.

CLSM, cleared and stained with basic Fuchsin according to Fuchs.
© Peter Huijser

Arabidopsis thaliana leaf - detail of chloroplast ultrastructure with grana, stroma thylakoids and enlarged plastoglobuli.

TEM after chemical fixation with aldehydes and osmium tetroxide.
© Ulla Neumann

Nuclei in Petroselinum crispum cultured cells.

Fluorescence microscopy after DAPI staining.
© Imre Somssich

Cardamine hirsuta exocarp - Golgi body enclosed in a transvacuolar strand.

TEM after high pressure freezing and freeze substitution. Immunogold labelling with JIM7 antibodies.
© Ulla Neumann & Angela Hay

Arabidopsis thaliana leaf expressing a WRKY transcription factor-GUS fusion protein.

Widefield microscopy, GUS staining detected in hydathodes.
© Imre Somssich

Arabidopsis thaliana flower - detail of the stigma.

SEM after cryofixation.
© Rainer Franzen

Arabidopsis thaliana root expressing WRKY27-GUS transgene.

Light microscopy image revealing nuclear localization of the transcription factor WRKY27-GUS fusion protein.
© Imre Somssich

Arabidopsis thaliana - overview of stem vasculature.

Light microscopy (bright field) of hand section. Lignin stained red with phloroglucinol-HCl.
© Peter Huijser

Arabidopsis thaliana - detail of stem vasculature.

Light microscopy (dark field) of hand section. Lignin stained red with phloroglucinol-HCl.
© Peter Huijser

Phloroglucinol crystals seen in the light microscope under polarised light.

Phloroglucinol-HCl is used to specifically stain lignin (Wiesner stain).
© Peter Huijser

Cardamine hirsuta seed - detail of the embryo.

Light microscopy after chemical fixation, resin embedding and staining of semithin sections with toluidine blue.
© Ulla Neumann & Angela Hay

Marchantia polymorpha gemmae - nuclear pore complexes.

TEM after chemical fixation with aldehydes and osmium tetroxide.
© Ulla Neumann & Peter Huijser

Arabidopsis thaliana root colonized by Colletotrichum tofieldiae.

Fluorescence microscopy after chemical fixation and resin embedding. Autofluorescence of semithin section.
© Ulla Neumann & Kei Hiruma

Colletotrichum orbiculare - detail of hyphal cytoplasm showing different fungal organelles in false color.

TEM after high pressure freezing and freeze substitution.
© Ulla Neumann, Richard O'Connell & Yasuyuki Kubo

Arabidopsis thaliana cotyledon - epidermis with stomatal guard cells.

CLSM - autofluorescence in red, ER membrane marker in green.
© Elmon Schmelzer

Cardamine hirsuta valve epidermis - detail of the cell surface.

TEM after high pressure freezing and freeze substitution.
© Ulla Neumann & Angela Hay
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